Abstract
Purpose. The aim of this study was to measure lipid profiles among severe and mild familial hypercholestrolemic (FH) patients and to screen unrelated families for LDL receptor (LDLR) gene polymorphism.
Methods. Fifteen FH patients and six controls were enrolled in the study. Total cholesterol (CH), triglyceride (TG), high-density lipoprotein (HDL-cholesterol), low-density lipoprotein (LDL) and ApoB-100 levels were measured spectrophotometrically. Patients were classified clinically into severe and mild, based on lipid profile phenotype and the presence of cardiovascular manifestation at the age of disease onset. Low-density lipoprotein exon 4 receptor gene polymorphism was screened by the SSCP method.
Results. FH severe phenotype patients had significantly higher total cholesterol levels (14.34 ± 1.14) mmol/L as compared with mild FH phenotype (10.13 ± 1.23 mmol/L) and experienced earlier symptoms in the childhood (9.25 ± 3.22) years as compared with those of mild phenotype, who did not experience symptoms until the mid of the fourth decade of life (36.7 ± 5.25 years). No significant difference was found between HDL-cholesterol, LDL-cholesterol, ApoB-100 among severe FH as compared with mild FH patients. All FH pedigrees showed the autosomal dominant mode of inheritance. SSCP results showed a wide spectrum of LDL exon 4 gene polymorphism among severe and mild phenotypes, which differed from control.
Conclusions. There are statistical differences between severe and mild phenotype FH patients in total cholesterol, triglyceride and the age of biological expression among FH Jordanians. FH patients showed a wide spectrum of gene polymorphism within the LDLR exon 4 gene. These polymorphisms were different among FH families; this may be related to founder factors, such as the degree of consanguinity among FH patients’ parents and shed light on the importance of the role of genetic screening and counseling in reducing the risk of occurrence of the new severe FH phenotypes that develop severe complications early in life and need LDL aphresis, as well as on the importance of cascade screening to trace and identify people at genetic risk, using family tracing, for an early diagnosis of the mild FH phenotypes that can be treated with lipid lowering therapy and life style changes early to prevent later cardiovascular complications.
Keywords: familial hypercholesterolemia, low-density lipoprotein, receptor, homozygous, heterozygous, SSCP, Apo B-100
Downloads
Download data is not yet available.
Most read articles by the same author(s)
-
Diana Bužinskienė,
Matas Mongirdas,
Saulius Mikėnas,
Gražina Drąsutienė,
Linas Andreika,
Indrė Sakalauskaitė,
Chemical peritonitis resulting from spontaneous rupture of a mature ovarian cystic teratoma: a case report
,
Acta medica Lituanica: Vol. 26 No. 4 (2019): Acta Medica Lituanica
-
Elija Januškevičiūtė,
Vaidas Vicka,
Justina Krauklytė,
Alvita Vickienė,
Donata Ringaitienė,
Mindaugas Šerpytis,
Jūratė Šipylaitė,
Acute Kidney Injury in Cardiac Surgery Patients: Role of Glomerular Filtration Rate and Fat-Free Mass
,
Acta medica Lituanica: Vol. 28 No. 1 (2021): Acta medica Lituanica
-
Vaida Gedvilaitė,
Diana Schveigert,
Saulius Cicėnas,
Cell-free DNA in non-small cell lung cancer
,
Acta medica Lituanica: Vol. 24 No. 2 (2017)
-
Vilius Rudaitis,
Gailė Maldutytė,
Uterine arteriovenous malformation treated with selective embolisation of uterine arteries: a case report
,
Acta medica Lituanica: Vol. 28 No. 1 (2021): Acta medica Lituanica
-
Andrė Lideikaitė,
Julija Mozūraitienė,
Simona Letautienė,
Analysis of prognostic factors for melanoma patients
,
Acta medica Lituanica: Vol. 24 No. 1 (2017)
-
Diana Bužinskienė,
Saulius Mikėnas,
Gražina Drąsutienė,
Matas Mongirdas,
Uterine sarcoma: a clinical case and a literature review
,
Acta medica Lituanica: Vol. 25 No. 4 (2018)
-
Elena Paškevičiūtė,
Diana Bužinskienė,
Kristina Ryliškienė,
De novo migraine with aura in the third trimester of pregnancy: a case report and literature review
,
Acta medica Lituanica: Vol. 28 No. 1 (2021): Acta medica Lituanica
-
Birutė Gricienė,
Monika Šiukšterytė,
Local Diagnostic Reference Levels for Paediatric Head CT Procedures
,
Acta medica Lituanica: Vol. 28 No. 2 (2021): Acta medica Lituanica
-
Behnam Baghianimoghadam,
Aidin Arabzadeh,
Yousef Fallah,
Ellis–van Creveld Syndrome in Iran, a Case Report and Review of Disease Cases in Iran, Middle East
,
Acta medica Lituanica: Vol. 28 No. 2 (2021): Acta medica Lituanica
-
Gerda Čėsnaitė,
Gintautas Domža,
Diana Ramašauskaitė,
Jelena Volochovič,
Diana Bužinskienė,
Factors affecting the maternal-foetal relationship
,
Acta medica Lituanica: Vol. 26 No. 2 (2019): Acta Medica Lituanica